Abstract:The pathotypes of four populations of cereal cyst nematode in Shandong,Henan and Anhui contiguous area were identified using 23 cultivars of international host differentials set (IHDS) and a local wheat cultivar Wenmai 19. The results indicated that the pathotypes of these populations were different from 16 pathotypes which had been nominated. Thirteen cultivars of group A in the IHDS had the same resistance reaction to Heze and Yingshang populations, they belong to the same pathotype. The virulence of Shangqiu population was similar to that of two populations above; barley cv. KVL191 was resistant to Heze and Yingshang populations, but Shangqiu population was virulent to this cultivar. The virulent band of Huaiyang population was broad than the other three populations. The local wheat cv. Wenmai 19 was susceptible to all of four populations.