Abstract:Laboratory incubation experiments were carried out to investigate effects of soil moisture and temperature on(nitrification) rate and N2O emission,and effects of nitrification inhibitor(N-Serve) incorporated with sand on N2O emission.The rate of nitrification was increased with the rising of soil temperature,with the highest rate at 30 ℃;soil moisture didn‘t affect the rate of nitrification significantly.N2O emission is increased with the rising of soil temperature and moisture.The highest N2O emission occurred at soil moisture being 28.5% and soil temperature being 20℃ or 30 ℃.At 30 ℃,N2O production was quite low under moisture stress(14.2%) with a cumulative N2O emission being 126.4 (mg/kg),which was primarily from nitrification;cumulative N2O emission was decreased by 65.0% when 80mg/kg N-Serve was added.Cumulative N2O emission increased rapidly with the cumulative emission being 764.4 mg/kg when soil moisture increased to 28.5% under which denitrification occured;however,it was decreased by 82.9% and 62.1%,(respectively) when mixing sand at a ratio of 1∶1 or adding N-Serve.We could conclude that applying N-Serve could(inhibit) nitrification at low soil moisture,while at the high soil moisture,applying sand or combine applying sand with N-serve could inhibit N2O emission efficiently.