从新疆阿克苏地区核桃园发病的核桃树上采集的病样经分离、纯化得到2种菌。经田间人工接种致病性测定,明确了引起核桃基腐病的病原菌。根据形态特征和培养特性初步鉴定核桃基腐病的病原菌为寡雄腐霉。采用真菌通用引物对分离的病原菌进行PCR扩增,核糖体DNA内转录间隔区测序,并将测序结果在GenBank中进行同源性比对分析,分子鉴定与形态鉴定结果一致。寡雄腐霉作为核桃基腐病病原菌系首次报道。
A new canker disease had taken place on walnut (Juglans nigra) in Xinjiang. In resent years, it has caused severe damage to the walnut product. In this paper samples were collected from diseased walnut in walnut gardens and 2 isolates were obtained. The isolates were inoculated with both wound and healthy inoculation methods. Re-isolation from lesions formed by the inoculation was the same as the inoculatied isolate, demonstrating that the isolate was a pathogenic strain of Pythium oligandrum based on its morphology. The sequences of the isolates Pythium oligandrum were amplified by PCR technique with universal primers of fungi, and the sequences were further compared with that in GenBank. The molecular identification confirmed the results from morphology identification. This is the first report that Pythium oligandrum is the pathogen of walnut black water disease in China.
全 文 :!!"
" !
#$
#
$ % # %
$
#$
%
& ( )
&()*+(, &(-.,) &(*(,)
./01!"!*/1#$
2345!$ % # %
QÌÈðU=UÜH^
"
ª
6
«
#
6
2
#!$
6
¨
#
¯°±
#
6
²³
#
6
?´µ
#
6
¶·¸
#
"#1
×GBD)&)$3u)G
6
yð<
9<%%8$& $1
ÈA&D)&)G
6
1Êñ
#8%%!%#
!
6
"
! 6
0×G*e1QÌ3«U=QÌX~=UõI<â
(
7Wæ3
$
iI
$
I9þ78TicU
a^
!
z@RD¸QÌÈðU=UÜI
$
[2±oUäðUz]H^QÌÈðU=UÜIQòpðæ
$
XY×IMYDô9<â=UÜIst
Yb
Ô
!
Qå
2*,
füþ-1a$
!
pÃa$wxî
?3C=FCW
Es
t\¨Þ9
òpðæQQÌÈðUUÜImÃý¦§
$
#$%
% 6
QÌÈðU
&
cU
&
±o)`
&
òpðæ
&()*
! &7"<1#8666
+,-./
! ,666
+01*
! #%%# :7!99"$%#%##$ :%%;7 :%!
ÃÄÅ#
% $%#% :%< :%<&
ÆÇÅ#
% $%#% :#% :##$
ÈÉÊË
%
FÌ
-
rly
.
(CÎÏÐÑÊË
"$%%7=,2<"=%<#&
×GxóÊÍI1ÒD(CÊ
"$%%7<##<" :$#&
×GxóÊÍI1(Cq
ÊË
-
xi1Ñ$BôþÜ!=LM
"$%%7<##<" :$#.
ZYH
-
ÞqÒ
"$%%7<##<" :$ :8#.$
"e
)
qÊQMOP
$
F*%1&8#.L3#.%)")$*%)&.&"0*+.C%/%*+U7&%H)-#*-#
&BFCDiHCD
#
6-H@ \@3M3CD
#!$
6,K0HEFLFJ
#
6YFC @CK3
#
6ABF/AB3CR@
#
6GFX/CDTHF
#
"#1NB6#$;-$"3+"#($#06-7 D"#$.:25$2#! O.-P.6-? )?#.:25$2#65,-./#(.$06,#2;R.9<%%8$&
$1+"#($#04:%""5! !"#$%6($+"#($#0,-./#(.$06D6#C.- #8%%!%#
9-%(*$%%6,C3Q4FCW3JKHE3FE3BFK LFW3C S0F43/C QF0C@L"T2?56-(-.?#6# HC \HCnHFCD5(C J3E3CLR3FJE! HLBFE4F@E3K
E3N3J3KFOFD3L/LB3QF0C@LSJ/K@4L5(C LBHESFS3JEFOS03EQ3J34/034L3K MJ/OKHE3FE3K QF0C@LHC QF0C@LDFJK3CEFCK $
HE/0FL3EQ3J3/PLFHC3K5+B3HE/0FL3EQ3J3HC/4@0FL3K QHLB P/LB Q/@CK FCK B3F0LBRHC/4@0FLH/C O3LB/KE5b3jHE/0FLH/C MJ/O
03EH/CEM/JO3K PRLB3HC/4@0FLH/C QFELB3EFO3FELB3HC/4@0FLH3K HE/0FL3! K3O/CELJFLHCDLBFLLB3HE/0FL3QFEFSFLB/D3CH4
ELJFHC /MJ0$%.2;"5.?6-7#2;PFE3K /C HLEO/JSB/0/DR5+B3E3U@3C43E/MLB3HE/0FL3EJ0$%.2;"5.?6-7#2;Q3J3FOS0HMH3K
PRYbL34BCHU@3QHLB @CHN3JEF0SJHO3JE/MM@CDH! FCK LB3E3U@3C43EQ3J3M@JLB3J4/OSFJ3K QHLB LBFLHC ?3C=FCW5+B3
O/034@0FJHK3CLHMH4FLH/C 4/CMHJO3K LB3J3E@0LEMJ/O O/JSB/0/DRHK3CLHMH4FLH/C5+BHEHELB3MHJELJ3S/JLLBFLJ0$%.2;
"5.?6-7#2;HELB3SFLB/D3C /MQF0C@LP0F4W QFL3JKHE3FE3HC BHCF5
:#6 ;&(3-% 6QF0C@LP0F4W QFL3JKHE3FE3& SFLB/D3CH4HLR& O/JSB/0/DH4F0& O/034@0FJHK3CLHMH4FLH/C& J0$%.2;"5.?6-7#2;
66
QÌ
"T2?56-(#?.6#
ó1`=DHxC
l
!
`®l<
!
iMî¨Û=TEJý
Î÷
!
©»µÛY»qiÛ
$
QÌ®óÒ
=YÝi
(
¼oi¼ô÷¨i
!
Umn
i§æ193
$
Ëgî×GÕGe1¼oÞß
JéBxðm«ÕþÒDY
$
°$¾
¢
!
þ×GQÌi§m5$ÔD
!
%£ôë
(
ZðÜ!=3!«¬3QÌ$biBô=þ
Ü!-Ü%
!
UV>GHÅqx
!
>?RQÌÿ
=D]ýþ«¬
$
ÅP
$%%9
$Zî*ÓôÚð
(
Î<
F¼-ÿ*+QÌU>=E«îQÌÈ/
I_xðâpáÅ;=~Y
!
õì1h«
!
6
ö1Iðâ
!
8<IøVÅ
!
&~Y$QÌð
âUzv=6!
%&
!
÷Uz]+QQÌÈ
ðU
"
î×GÕGe1ÓQÌÅ;U
#$
2*+
!
$%%9$%%;
$QÌÈðUî*e1I
81;!`!
fcQÌ&÷¹º
!
ãÒ
>?R·eQÌÿ=«¬
!
²QBxÃIJ
«¬=»½%v
$
)j´U>
!
F<@QÌÅ;U
"
´÷
U
#
î_DH
(
±F
(
¹ºà@5à_ï«
$
#9<9
$¹ºàÃý«´÷U
!
¤_î
#7$"
$@
5à9&U³¦§
"=JFEH3J!$%%!#$
´÷U=U
ÜîáâQæ`
"J%0$"B%$%"#6 #
=
JA
:6;C./"#6
JA:.--6;";.& #;<9
$nFø«j
´~Y
!
h@^UÜIQ
JA:6;C./"#6".FCCHCH$
65!$%%##$
F
<@Å9/dUóî
m=×ûü
& ( )
!"
"
6
U>
"
gú¼-
!#;;7#!
·U>$QÌÈðU~Y
ð´
!
J%0$"B%$%"#6 :6($6-6
D¸
$
ðæID¸
=±ÐÜUît1`mn<.
!
S=ó±ø,
±%ÐÜU8tI¹º
!
§h6a¹º
$
§/
UÐÜUIQ>h
!
MSyî5y~=X¹
9
!
oxU9D8ub
!
©¾Þù§/c¹º
!
îï¼½b$=
Gæ
!$%%9#$
ËPQÌÐ(ãÒð>+:é
øLD¸¼ë=áÅ;
$
%&
!
<âH
^QÌÈðUUÜIîÒ=_`
$
K¥=UÜ
)Oa°±óMv´K`UÜI±oUä
!
¾
@^U>jk
$
¤ðæ=ð´U=ð3
è
!
8±oUä¾`3
!
H^8×¢
~D
!
%8
$
Me[2QÌÈðU«U~Y
(
UÜI±oU
ä
(
¼ô)UUÜI=Qå
2*,
füþ-
"HCL3JCF0LJFCE4JHP3K ESF43J!(+
=$n-9QÌÈ
ðUUÜIìMstRH^
!
úîLMQÌÈðU
UÜI
!
Q»^kêI¸¹[ñÆ2
$
#6
Ýr$°±
<=<>
ÿ!
#1#1#6
ãä;åæ
6$%%9
$
#%##
%0×G
*e1ôÚðÿ
(
Î
µ&Û
È/<
!
×Ç¥²áY
$
#1#1$6
çCÉ
6
zÎÂðÈ
",#(
ûÌðÈ
"pG,#(
ÙÚ÷ðÈ
"+,#(
fÛZðÈ
(
&
jðÈ
"G,#( Y2,
ðÈ
(
1û|
" S3LJHE
E/0@LH/C!Y(
ÖnZù¼|
"
üý;
v
{;Q
#v##"
ÿ8
! #;;8#$
#1#1<6
è6
6
×IQåÈ%füþ-1
(+
"8 j^+?+,??+?,,+???j< #^Z(+&! " 8 j^++
?++,++?,+,+?j< #^$
<=?>
?@=>
#1$1#6
áéêëìí
6
î×G*ôÚðÿ
(
Î
^#
(
^Ó`«UQÌî3e=y~Y
!
p
ÿsûü
$
#1$1$6
áî¢;AïS
6
YSefg<â°±
!
áYÙÚðÈst<â
!#8 m
$8 m
ð
!
7Wh9<âôst9j
(
RMfôJö
$
#1$1<6
ðá89D
6
cUa^XYá~T
iÀ~Ti
$
i°±
"
°E¨
!#;;9#$ ##
Ti
%
Ã
Y2,
ð
<
=<âô
!
µIþ
"
.
" OO#
T
#8% O-
|
Y2,
ðÈ
îúgýQð
8
Y
$ $#
~áTi
%
YL¤uÇ¡/y
1
!
ËYÿ
78`
!}=¤uÁ=/
!
N!}Õ
«h
!
Yà1Ñ~y1
!
Y
8% O-
<âôx|L
¤u
!
"TiÓhYRz¥×ÝRTiÓ
!
®
;<|â
!
N
!9 B
hµ
$
9"Y\õ°±TT
8% O-
ÀI;
!
úÝßë
!
ýÒ"
$ <#
À~Ti
%
T
ißymÁ=
!
Y
8% O-
<âôx|L¤u
!
Ý
Rî/ym
!
hYRz¥×ÝRTiÓ
!
9"Yõ°±TT
8% O-
ÀI;
!
úÝßë
!
ýÒ"
$ !#
*+¥Ð°±
%
Ti«U
"`# c
Ti«UÓ
Z
TiÓ
a#%%`$ 8#
cU²
%
[2.#û±
!
9«U=QÌ1=UÜstË<â
!
v´K
`<âæ3=UIó]$ÜTiIlc
$
#1$1!6
áî¢ñÖJKBçC8ëìí
6
Ã<â
æ3=IiT3
G,
ðÈ
!
$8 m
ð
<
!
ûüI®±o
&
ð
8
hKOmAQ
(
k$Z-
Qh=ÿ¼]Z
$
î&\
!
ù¼|
"
üý;
v
{
;
c#v##
ET1
# 4O
$
=QÌ1
"
þÿ
#% OHC#!
ÃI:Tù¼|
Y&
|E
!$% m
ð
7 _#%
!
%µI?`Z&ùf]Z
"
+h-
!
#;;!#$
#1$186
áî¢;AòD
6
Y×IMYDô
(+Z(+&!
9UÜIst
Yb
Ô
!
9¤æ=ÿô
sta$
!
Ãæ3=$nî
?3C=FCW
EstÞ9
!
M\¨
##
UÜI
2*,
[µ
6
ÃUÜITi3|
ðÈE
!
îõýEð
8
hÃ~I?
$
XY
+,=
±[µ7WÈ%f
2*,$
$#J2*,(+&
=Ô$$na^
6
XY×IM
YDô
(+Z(+&!
Ô
!Yb
m5
$8
0
-!
|Q
% =&,$18
0
-& #% aYbP@M3J$18
0
-!$8 OO/0)-
:#
GD0
$
8
0
-!$18 OO/0)-
:#
K*+Y
#19
0
-!8 [)
0
-
:#
E6R
á
%1#8
0
-!
Ü<
2*,< CD!
Dô
(+
(+&!"
»
&FCD/
Iê²
#
q
#
0
-
"$8
0
O/0)-
:#
#!
T
KKX
$
p
å5¨3
#$1%8
0
-!
î
Yb
Ô[stÔ
$
Ô3è
%
;! m
3V
< OHC& ;! m
V
# OHC!88 m
]¡
# OHC!7$ m
¿À
# OHC!
<8
ÝùÞ
&
úh
7$ m
¿
À
#% OHC$
$na^±ô»¼8¼ô8CÙE
êµIa$
$
< #
U Ü I
J2*,j(+&
$ n î
*=(
¾ ³
"BLS% ZZP0FEL5C4PH5C0O5CHB5D/NZ=0FEL54DH#
E=ð
)$n=
(+&
1st\¨Þ¨
$
9;
6!
#$
# (
6
)-
%
QÌÈðU=UÜH^
$6
wx$
¤H
·U>xî<=/
!
/ÈI«
!% 4O
=PQ
©xU9
!
/È
" _#% 4O
ß
¨b
!#
/©ÿ¼
#
3bÝU9
$ !
%)¼y
%E)
!
î«Uz#
!
1ymo6x~Y
!
îö1
I<IA`ßðâVÅ
!
þg==Û
!
U9
Ô¬
!
1°$<Iõâ
!
UI1°¢£«ì
K
!
KáßDuŽ|áx
"
ª.#
:,!=#!
9
%|áúb
!
9/st£
(
¡ô«ö1IV
ÅX¹
!
<IøVÅ
!
p««¬
!
ãÒ<
ItIVÅ
!
UýA`ß8½9Þzv
$
«Uh
#
!
U]¦Ô¬¼[I
!
[¢½ðâ
!
î/È
IU9Ô¬¼l@
!
fcâl
!
3/
d
!
úh¹º
$
U>î
#%
%)£®«¬
!
¼!
$
$
8
%îq$U9=Ò«Ô¬
!
áx½
|
$
?=?>
¾t(Å£
9Ufg<â7Wh
!
æ3
$
iUÜII/
!
<
VQ*`
"+2(6#.2; ES5#
ðæ`
"J0$%.2;
ES5#!
RMQ
-#
f#$
?=@>
8<
$%%;
$
!
%
#8
Å
!
Iá
<âð=
$
i
Ist9þûTi
!
wxy
#$ -#
IiÀcU
!f#
IiîcU
"
ª#
:!2#$
`
<>?
b¾ttß~bªü
!
A*9B<>7#-C+%&").&$C+*%).8 %;& )-&+*%#-).")#+3
Ti°!
(C/4@0FLH/C O3LB/K
cUUÜI
YFLB/D3CR
«UÓ
(C4HK3C43
«U
(C4HK3C43JFL3Z`
À~
(CLF4LC3EE
ðæI
J0$%.2; % %
*I
+2(6#.2; % %
ðæI
d
*I
J0$%.2;d+2(6#.2;
% %
o % %
~
>/@CKE
ðæI
J0$%.2; #9 ;%
*I
+2(6#.2; % %
ðæI
d
*I
J0$%.2;d+2(6#.2;
% %
o % %
66
"úißëTi
$%
ÝÓ
$ )F4B LJ3FLO3CLBFE$% HC/4@0FLH/C
ES/LE5
66
~1TiE
!-#
ÀcU
!f#
=I/î
cU
!
Ti
#!
h«U¨3R
;%`$
Tih
!
#!
îTiÓ£«
(
¡«Ô¬zv
!
£«Ô¬
$ _#% OO!
¡«Ô¬
%18 _! OO!
hÔ¬
!
!
$%
!
£«Ô¬¨3
9 _#8 4O!
¡«Ô¬3
! _
7 4O$
!
<%
1°$<I<â
!
<IV
!
áxÀu½|
!
!
<8
÷|¨b
$
9"~1ß
ÀÔ¬i
!
~á
#
@V
!
TiÓ¾1°f
gQl½
$
0«U/U9UýATß<âæ3$T
iIð\=ðô
!
RM
f$!
2&¾Q
f#
QQÌÈ
ðU=UÜI
!
UI©M~áT
$
$%#%
$
!
%
<%
Å`Ti©²e
!
~Ti
°±EU9î
$
ÝTiÓCþÔ¬zv
!
£«Ô¬
#% 4O!
¡«Ô¬
8 4O!
ö1I$<Iõâ
!
Cþ
Du5;
$
?=D>
¾t]Å£H
$
EHI
î
G,
ðÈ
!
z#Àzv=h¼I?
!
h
#h¼I?²NYTðÈym
$
I®«Y
!
@Òë+
!
ë½
!
ðÈm8½lc
!
À½v<
ï
$
¼½Q?}ż½
$8 _<% OO"
ª.#
:
)!f#$
I??}
<1% _"
0
O&
I?,Du±¼
u±rY,D
!
S·¼
$
Z&u±¼u±
!
À
I<±Y6e
!
S
I?ç²"ê
$
mAQ
u±
!
w¼þ¼
!
t¼þt¼
!
?}
#8 _
<%
0
O"
ë
$#1$
0
O#&
mAQ-î.±q¸ô
!
q¸ôÛ
$18 _719
0
O"
ë
!1$%
0
O#!
ÈI·
#18 _$18
0
O"
ë
$
0
O#$
pQ¨À±²
!
±²
¢
/±å0±
!
r?¼
!
¾wÌtm$mAQ
T|
!"7 _#9#
0
Oa"< _#$#
0
O$
AZu±
!
ë
+
!
6¤ßmAQ°eßQ
!
?}
#< _$9
0
O"
ë
$%
0
O#!
-k
#1! _#19
0
O"
ª.#
:?!X!(#
"
Ä$
!#;;9&
ÀB¯
!#;7;#$
ª
#6(+&
1
Yb
Ôÿô
fHD5#6YbjFOS0HMH3K SJ/K@4L/M(+& J3DH/C
G52*,OFJW3J& #1Yb
SJ/K@4L/Mf#ELJFHC& $1YbSJ/K@4L/Mf$& <1o5
?=Q>LA/
4/0(
I
f$
÷Ô
æ3·l1Û
!
8Y;QM9"=
o
^÷
Ô3·1Û
$
M9
(+&
1=««a$
!
¤æR
½Q
98% PS
=
J2*,j(+&
È%$n
!
Ã&$n$
;;
& ( )
!"
"
6
?3C=FCW
E=$nst\¨Þ¨
!
wxyzQÌ
ÈðUUÜ$òpðæ
"J0$%.2;"5.?6-7#2;#
\¨
Û¨
;;`"
.üM
?[<$<?=X>
¾t]<
[2U>~Y
(
UÜðU
(
±oUä
(
cU
a^
(+&
È%$n\¨9Þ-stkê<
v
!
Æ)qr¾Q
!
D¸QÌÈðUUÜQò
pðæ
$
?=Y>
; b¡ß]78
[2±oUä$qrNj=UästÞ9
!
JA"5.?6-7#2;
JA6-6-7#2;
=1VógP
Z&u±I?YwkcwuYwk\f²"
ê
!
hPZ&1Ô±½±
!
MSîl2q
$
$
JA(.-(
=1Vó
JA(.-(
Z&u±
!
6
±²"ê
$
$
JA6;6(:25.-2;
=1Vó
JA
6;6(:25.-2;
pQ^
"
Ä$
!#;;9#$
I©²Næ
!JA"5.?6-7#2;
I?¼½=úO
(
ú¶
(
úÛg
!% m$
$eNû
Ê=úÛ¼½g
!! m
xT
!JA(.-(
JA
6;6(:25.-2;
=úÛ¼½g
Ä
$
! #;;9#$
<6
w$P
MeÃýÃ×G*e1QÌÈðU=UÜ
IH^Qðæ`
!
p[2U>~Y
(
UÜðY
(
±o)Uä
(+&
$n\¨Þ¨Ã·UÜI@^
Qòpðæ
!
z@R·UIóD¸QÌÈðU=U
ÜI
$
î×GmDQÌÿ1b$¾¢Ã·Ub¾Q
QÌðâU
!
îe·EèQÌðâU®ÝQ
ÌÅ;U
"
ë30
! $%%%#$
4P=1Vó
%
Ãq
!$
iU>=~Y6!
QÌðâUE>/=1
°
!
1°mÿ¼ÅÓ
!
häL
!
<¼ZQ
5x6Ľ<¼Z
!
U9Ú/=£««¬
!
h#UI1°£K
!
áxDuÅ;
$
îD/
!
U9z#+mîö1I
!
;bU9¢EYW
ð7c
!
lY0yo6xzv~Y
!
·1°8x
-
Å;
.
!
1ïÔ¬Q¨D=U9
$
îD/
x=~Y$î*e1«¼=QÌÈðU=
~Yð!
U9ßøáxDuÅ;
!
¤ãÒö1
I
(
<IVÅðâ
!
îU9«Uh#ÀÅÓ<
¼Zÿ¼
$
ý
!$
iU>=UÜI6!
QÌ
ðâU=UÜQ
90$"(B"#6 P2?56-7.(!
8MLM<â
Næ3=QÌÈðU=UÜQ
J0$%.2;"5.?6-7#2;$
½#¾¢
!
ÃQÌðâU$QÌÈðUQl9
!
f
0UÜ1$
iU>
!
fc½#öî:b
¾Õ
$
òpðæD¸=QÌÈðUîF
^¦
§
!
æ`
"J%0$"B%$%"#6#
D¸=U>ð´=~
Y
$
seú-
"$%%$#
¦§
!
p1éðUóÕm
=li×ûüU>
!
UÜIóàæ
"J%A
:6:$"#2;#&
+©;-
"$%%%#
¦§
!
l@m<=Ì
æU=UÜIøQàæ
$
MLMstUÜI<â
!
^<â3æI
!
¤<â3*I
!
9&UÜI
st·ÔðhTi3ýþ§/
!
§/pf«
U
!
tzý6óD¸QÌÈðU=UÜI
$
Ã*
I$ðæIêTi3ýþ§/
!
§/pf«
U
!
tzø6ó
$
iI"êcNc
$
HM©
²X~=U>ìM
µ*e1=QÌÿ
1
!
e°ó]Q\jk=U>
!
ô9U>«¼
=PQstmn*+
$
(+&
ów
#9& J2*,!819& J2*,
$9& J2*,
Cþ=1Ù
!
·1ÙsW¨R/1
!
ï=L
Myz
(+&
î×I=iþöîþ}=Vr
!
8î
i
6\I/þLÛJ]
!
©¾Q×I=m¥«
MLMXY<
Z¼ô)°±9QÌÈðUUÜ=Qå
2*,j(+&
$nst
N<âIi
f#
=Qå
2*,j(+&
$n$ðæI=\¨úÛ
!
$òpðæ=\¨
¨
;;1%`!
0
$
n o + ,
ë30
5$%%%5
QÌðâU=«¼$I
5
l@&(C
! "##% <9 :
!%5
Gæ
!
-G
5$%%95
±<ÐÜULMs¬
5
åA&(C
!""#
<9 :!#5
gú¼
!
6
/
5#;;75
î
<@U>×ûü
5
î
BD))
¦
! $! "!#% <$7 :<<#5
seú
!
øÄØ
!
%Äî
!
-
5$%%$5
p1éðUUÜILM
5
Õ
BD))¦
!#7"$#% #!< :#!"5
°E¨
5#;;95
§ULM±
5<
.
5
A -
%
EFBx.K
5
l@xLMN
5#;9%5
QÌ
5
A-
%
EF&x.K
!8" :"%5
+h
!
ûtî
!
\h²
!
-
5#;;!5
m¼&j=
"
iðæcU
LM
5
§ôUë)¦
! "!#% +©;
!
güù
5$%%%5
l@m<=ÌæU=^7UÜH^
5
@
ABD))¦
! <%"$#% #;% :#;#5
ÀB¯
5#;7;5
×IH^v>
5
»
%
»()CÙx.K
!<% :Ä$
5#;;95
EF×Ii
%
!
"
"ÁæË
5
A-
%
()x.K
! 88 :"85
ÿ8
5#;;85
æILMCÙ
5
A-
%
EFBx.K
! # :9"5
=JFEH3J5$%%!5YJ/DJ3EEHC J3E3FJ4B /C J%0$"B%$%"#6 KHE3FE3E/MM/J3EL
LJ33E5f/J3ELb3E3FJ4B! # :!5
=JFEH3J!i@CD+5$%%#5b343CLK3N30/SO3CLEHC J%0$"B%$%"#6 KHE3FE3E/M
LJ33EFCK CFL@JF034/EREL3OEHC )@J/S35YBRL/SBLB/JF2HE3FE3E!8 :#"5
.FCCHCH,! .3LJFHC/,G5$%%#5(CW KHE3FE3HC 4B3ELC@LE% HOSF4L/C
LB3)@J/S3FC 4B3ELC@L5f/J&C/Q-FCKE4b3E! 7""<#% !
!"#$
6
ÁÂÃ
"
%%#