Abstract: A protocol for extracting chlorogenic acid from Eucommia ulmoides leaf was optimized by orthogonal test on the base of noticeable factors test. The preventing effect of extract of Eucommia ulmoides leaf on mice suffered from high lipid serum was evaluated. High lipid serum was induced by gavaging fat emulsion to mice everyday. The TC,TG,LDL-C and HDL-C level in the serum of different animal groups were monitored. The result showed that the optimum process was to extract the chlorogenicacid at 90 ℃ with 70% ethanol for 2 h, with solid-liquid ratio 1∶16,and the extract rate was able to reach to 4%. The extract was efficient in defending high lipid serum.
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