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作 者 ：汪星;潘重;马爱辉;赵开弘;周明;

期 刊 ：武汉植物学研究 2010年 28卷 4期 页码：480-485

关键词：别藻蓝蛋白；体内重组；相互作用；双杂交；

Keywords:Allophycocyanin, Reconstitution in Escherichia coli, Interaction, Two-hybrid,

摘 要 ：进行了Anabaena sp.PCC7120的别藻蓝蛋白ApcE与ApcF的体内重组的光谱和apcE和apcF基因的细菌双杂交的研究。在提纯前PCB-ApcE/PCB-ApcF的吸收及荧光光谱峰与PCB-ApcF的峰位置一致,而提纯后PCB-ApcE/PCB-ApcF的吸收及荧光光谱峰与PCB-ApcE的峰位置一致,表明ApcE与ApcF不能形成复合物。pBT-apcF与pTRG-apcE的转化细胞能在无3氨基-1,2,4三氮唑(3-AT)的非选择性培养基上生长,而不能在有3AT的选择性培养基上生长,说明在转化过程中未产生能抗3-AT的HIS3报告基因,进一步证实ApcE与ApcF间没有相互作用。

Abstract:We conducted a study on allophycocyanin proteins ApcE and ApcF from Anabaena sp.,PCC7120 bound PCB in Escherichia coli and two-hybrid bacteria system of ApcE and ApcF.Results showed that the λ_max of absorption and fluorescence spectra of PCB-ApcE/PCB-ApcF were the same as the λ_max of PCB-ApcF before purification,and the same as the λ_max of PCB-ApcE after purification,indicating that ApcE and ApcF could not form a compound.While the transcription of pBT-apcF and pTRG-apcE could grow in the non-selective growth medium,they could not grow in the selective growth medium containing 3-amino-1,2,4-triazole(3-AT).The HIS3 reporter gene did not engender during the transcription,indicating there were no interactions between ApcE and ApcF.

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