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作 者 ：谭荣,王毓杰,张雨欣,周礼仕,谭尔,阿萍,张艺

期 刊 ：中国中药杂志 2014年 39卷 6期 页码：1054.

关键词：藏药；风毛菊；HPLC；含量测定；质量控制；

Keywords:Tibetan medicine, Saussurea hieracioides, HPLC, content determination, quality control,

摘 要 ：该文建立了HPLC同时测定藏药风毛菊中茵芋苷、东莨菪苷和伞形花内酯3种香豆素类成分含量的方法。采用Wondasil C₁₈-WR色谱柱（4.6 mm×250 mm，5 μm），以甲醇（A）-0.1%磷酸水溶液（B）为流动相，梯度洗脱，流速1.0 mL·min^-1；检测波长325 nm；进样量10 μL；柱温35 ℃。结果茵芋苷，东莨菪苷和伞形花内酯在40 min内均达到基线分离，各成分在其线性范围内均呈现良好的线性关系，线性范围分别为0.18~5.6 μg（r=1.000 0），0.060~1.8 μg（r=0.999 9），0.032~0.97 μg（r=0.999 8）。平均加样回收率分别为99.16%（RSD 0.41%），100.3%（RSD 0.79%），102.2%（RSD 0.87%）。该方法操作简便，准确，重复性和稳定性较好，可作为藏药风毛菊的质量评价提供科学依据。

Abstract:This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of skimmin, scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C₁₈-WR column (4.6 mm×250 mm, 5 μm) with methanol (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at a flow rate of 1.0 mL·min^-1. The detection wavelength and column temperature were set at 325 nm and 35 ℃, respectively, and the sample size was 10 μL. The results showed that skimmin, scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 μg (r=1.000 0), 0.060-1.8 μg (r=0.999 9), 0.032-0.97 μg (r=0.999 8) for skimmin, scopolin and umbelliferone, respectively, with the average recoveries of 99.16% (RSD=0.41% ), 100.3% (RSD=0.79%), 102.2% (RSD=0.87%). The method is simple, accurate and reproducible and can be used for the quality control of S. hieracioides.

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