目的：观察三七总皂苷对急性心肌梗死(acute myocardial infarction，AMI)大鼠骨髓干细胞(bone marrow stem cells，BMSC)动员的效率。方法：140只Wistar大鼠建立急性AMI模型后随机分成：西药动员组(皮下注射G CSF50 μg·kg-1·d-1；假手术组和模型组皮下注射生理盐水50 μg·kg-1·d-1；中药动员组腹腔注射血塞通150 mg·kg-1·d-1；中西药动员组皮下注射G CSF50 μg·kg-1·d-1和腹腔注射血塞通150 mg·kg-1·d-1，除假手术组外，各组又按1，7，14 d 3个时间点分为3个亚组，其中G CSF每天1次，共用7 d，血塞通注射液每天1次，直到每亚组处死动物前停止, 采用流式细胞仪检测外周血中C kit+细胞量和全自动血细胞分离机观察白细胞(white blood cell，WBC) 计数和MNC(mononuclear cell，MNC)百分比。结果：模型组与假手术组1，7，14 d比较WBC、MNC%明显升高(P<0.05)；在1，7，14 d中药组、西医药组与同期中西医药组相比明显低(P<0.05)；西药组、中西医药组与模型组相比外周血单个核细胞C kit受体表达明显升高(P<0.05)，中西药组与各组相比变化明显。结论：三七总皂苷可协同G CSF更好动员BMSC，提高BMSC出髓效率，增加外周血中BMSC数量。
Objective：To study the mobilization efficiency effect on bone marrow stem cells by Panax notoginseng on acute myocardial infarction in experimental rats. Method: One hundred and thirty wistar clean rats with average age 16 weeks weighing (200±10) g, half female, ligated anterior descending coronary artery proximal，were established animal model of acute myocardial infarction. They were randomly divided into: model group, Chinese medicine mobilization group, western medicine mobilization group, integrated Chinese and western medicine mobilization group and sham group. Except sham group rats, other groups after the survival of 24 h by 1, 7, 14 d, and other points in time were divided into three subgroups. They were injected drugs immediately 24 h after the surgery. Injected G CSF 50 ｕg·kg-1·d-1 in western medicine group subcutaneously, NS 50 g·kg-1·d-1 in sham group and model group.In Chinese medicine mobilization group intraperitoneally injected notoginsenoside(150 mg·kg-1). Chinese and western medicines mobilization group were subcutaneously injected G CSF 5 μg·kg-1·d-1 and intraperitoneally injected notoginsenoside(150 mg·kg-1)，with G CSF once a day, 7 days totally and G CSF once a day until each sub group of animals were killed. Severn rats were remained for experiments in every subgroup. Flow cytometry were used to detect the percentage of peripheral blood mononuclear cells and white blood, C kit receptor expression of peripheral blood mononuclear cells. Result: WBC, MNC% of model group 1, 7, 14 d were significantly larger than those of sham group 1, 7, 14 d ( P<0.05), which indicates that MI animals can mobilize themselves. WBC and MNC% of Chinese medicine group and western medicine group were lower than those of Chinese and western medicine group in 1, 7, 14 d (P<0.05). C kit receptor expression in PBMC of Chinese and western medicine group and western medicine group were higher than those of model group. Western medicine is better than Chinese medicine in mobilizztion. Conclusion: Panax notoginseng may cooperation G CSF with mobilizing stem cell, raise the efferens efficiency of bone marrow stem cells, increase the number of peripheral blood stem cells.