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作 者 ：殷文光,李曼玲,刘淑芝,康琛,王彦礼,周钟鸣,仝会娟

期 刊 ：中国中药杂志 2009年 34卷 22期 页码：2946.

关键词：复方吴茱萸巴布膏；吴茱萸碱；吴茱萸次碱；固相萃取；高效液相色谱；

Keywords:cataplasm, Wuzhuyu, evodiamine, rutaecarpine, solid-phase extracttion, HPLC,

摘 要 ：目的：建立大鼠血浆中吴茱萸碱和吴茱萸次碱的SPE-HPLC测定方法，并用于透皮给药后的药动学研究。方法： Kromasil C₁₈(4.6 mm×250 mm， 5 μm)，以乙腈-水-四氢呋喃-冰醋酸（51∶48∶1∶0.1）为流动相，流速1 mL·min^-1，检测波长为225 nm，柱温35 ℃，以氯氟舒松为内标溶液，固相萃取后采用HPLC法测定吴茱萸碱和吴茱萸次碱的血浆浓度。结果： 透皮给药后，药-时曲线符合一室模型，吴茱萸碱主要药动学参数K_a 0.224 h^-1，K_e 0.114 h^-1，C_max0.211 mg·L^-1，T_peak 6.132 h，吴茱萸次碱主要药动学参数K_a 0.220 h^-1，K_e 0.118 h^-1，C_max0.272 mg·L^-1，T_peak 6.102 h。结论： 本方法简便、准确、灵敏度高，适用于大鼠透皮给药后吴茱萸碱和吴茱萸次碱的血药浓度测定及其药动学研究。

Abstract:Objective： To establish a SPE-HPLC method for the determination and pharmacokinetic study of evodiamine and rutacarpine in rat plasma. Method： A Kromasil C₁₈ column (4.6 mm×250 mm， 5 μm) was used with acetonitrile-water-tetrahydrofuran-acetic acid (51∶48∶1∶0.1) as a mobile phase and at a flow rate of 1 mL·min^-1， and the UV detection was at 225 nm. The column temperature was 35 ℃. After the analytes were extracted from the plasma of rats by solid phase extraction (SPE)， the content of evodiamine and rutaecarpine was measuared by HPLC method using halcinonide as an internal standard solution. Result： After transdermal administration to rats， the pharmacokinetic behavior of evodiamine and rutaecarpine belongs to the one-compartment model. The main pharmacokinetic parameters was as follows： K_a 0.224 h^-1 and 0.220 h^-1， K_e 0.114 h^-1 and 0.118 h^-1， C_max 0.211 mg·L^-1 and 0.272 mg·L^-1， T_peak 6.132 h and 6.102 h， respectively. Conclusion ：The method in this study is simple， rapid and sensitive. It is proved to be suitable for pharmacokinetic study of evodiamine and rutaecarpine.

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