Abstract:Objective： To investigate effects of Akt and ERK1/2 signaling pathways on waltonitone (WT) induced cell growth inhibition in human hepatocellular carcinoma BEL-7402 cells. Method： Cell viability of BEL-7402 cells was examined using MTT assay. Phosphorylation of E Akt and RK1/2 were detected by Western blot analysis， while cell cycle distribution of BEL7402 cells was analyzed by flow cytometry. Result： WT could inhibit the BEL-7402 cells growth， induce the S-phase cell cycle arrest， activate Akt and ERK1/2 phosporylation. Moreover， the cell growth inhibition and S-phase cell cycle arrest induction of WT on BEL-7402 cells could be blocked by Akt and ERK1/2 inhibitors. Conclusion： WT induce the cell cycle arrest and inhibit the cell growth on BEL-7402 cells by modulating Akt and ERK1/2 phosphorylation.