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期 刊 ：中国中药杂志 2008年 33卷 13期 页码：1609.

关键词：黄芪甲苷；液相色谱法；单向灌流法；肠吸收动力学；

Keywords:astragaloside Ⅳ, HPLC, single-pass intestinal perfusion, intestinal absorption kinetics,

摘 要 ：目的：研究黄芪甲苷的在体肠吸收机制。方法：采用大鼠在体单向肠灌流实验，利用HPLC法测定黄芪甲苷的含量，分别研究吸收部位、药物浓度、P-糖蛋白抑制剂对黄芪甲苷吸收的影响。结果：各肠段的吸收速率常数(K_a)和表观吸收系数(P_app)有显著性差异(P＜0.05)；在20～80 mg·L^-1内，小肠吸收速率常数和表观吸收系数无显著性差异；P-糖蛋白抑制剂对黄芪甲苷的肠吸收影响不大。结论：黄芪甲苷的肠吸收为典型的被动扩散吸收机制。

Abstract:Objective: To investigate the mechanisms of intestine absorption of astragaloside Ⅳ in rat. Method: The K_a and P_app of astragaloside Ⅳ was investigated using single-pass intestinal perfusion technique in rats. HPLC was used to determine the concentration of astragaloside Ⅳ. The effect of absorption site, drug concentration and the inhibitors of P-glycoproteon on the absorption had been studied. Result: By the testing of the statistics, the K_a and the P_app values of the duodenum, jejunum, ileum, colonic had significant differences (P＜0.05). The concentration from 20-80 mg·L^-1 had no distinctive effect on the K_a and P_app of small intestine. The inhibitors of P-glycoproteon had no distinctive effect on the absorption of small intestine. Conclusion: Astragaloside Ⅳ is absorbed by typical passive diffusion mechanism.

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