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期 刊 ：中国中药杂志 2006年 31卷 05期 页码：386.

关键词：西洋参叶皂苷；降解物；20(S)-人参皂苷Rh2；RP-HPLC；

Keywords:saponins from leaves of Panax qinquefolium, hydrolysis product, 20(S)-ginsengnoside Rh), RP-HPLC,

摘 要 ：目的:对西洋参叶皂苷碱降解物中20(S)-人参皂苷Rh2进行含量测定。方法:采用RP-HPLC,ZORBAXEXTEND C₁₈柱(4.6 mm×250 mm,5μm),流动相甲醇-水(85∶15),流速1.2 mL.min^-1,检测波长203 nm,柱温25℃。结果:20(S)-人参皂苷Rh₂的进样量在0.5～25μg有良好的线性关系,r=0.999 9;平均加样回收率为99.7%,RSD1.0%。结论:该方法测定20(S)-人参皂苷Rh2简便快捷,准确度高,测定结果证实该降解方法20(S)-人参皂苷Rh2的转化率高。

Abstract:Objective: To determine 20(S)-ginsengnoside Rh₂ in the hydrolysis product of saponins from leaves of Panax qinquefolium.Method: The separation was performed on ZORBAX EXEND C₁₈ column(4.6 mm×250 mm,5 μm),eluted with methanol and water((85∶15)) as mobile phase with the rate of 1.2 mL·min^-1at 25 ℃,the wavelength for measurement was 203 nm.Result: [WTBZ]The calibration curve was linear in the range of 0.5-25 μg for 20(S)-ginsengnoside Rh₂(r=0.999 9,n=7).The average recovery was 99.7%(RSD=1.0%). Conclusion: This method is simple,accurate,reliable and reproducible.The result shows that the transform ratio of 20(S)-ginsengnoside Rh₂ is high by this hydrolysis method.

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