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期 刊 ：中国中药杂志 2007年 32卷 12期 页码：1184.

关键词：紫红獐牙菜；RP-HPLC；含量测定；

Keywords:Swertia punicea, RP-HPLC, determination,

摘 要 ：目的：建立反相高效液相色谱法测定紫红獐牙菜药材中3种苷类成分含量的方法。方法：色谱柱为Alltimal C₁₈柱(4.6 mm×250 mm,5 μm),流动相为甲醇-水(水中含0.05%磷酸),梯度洗脱,流速1 mL·min^-1,检测波长254 nm,柱温30 ℃。结果：龙胆苦苷(gentiopicroside),芒果苷(mangiferin),8-O-β-D-吡喃葡萄糖基-1,5-二羟基-3-甲氧基酮(swertrianolin)分别在31.3～281.7,0.31～2.78,0.55～4.91 μg内线性关系良好,相关系数分别为0.999 6,0.999 3,0.999 5；平均回收率分别为103.36%,101.42%,97.39%,RSD均小于 3%(n=5)。结论：该方法操作简便,分离度好,为完善紫红獐牙菜药材的质量标准提供了依据。

Abstract:Objective: To develop a RP-HPLC method for determination of three glycosides in Swertia punicea. Method: Chromatographic column: Alltimal C₁₈(4.6 mm×250 mm, 5 μm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution.Flow rate: 1 mL·min^-1. Wavelength: 254 nm. Column temperture: 30 ℃. Result：The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 μg, (r=0.999 6, 0.999 3, 0.999 5). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n=5). Conclusion: It is a simple and sensitive meathod in controlling the quality of S. punicea.

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