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期 刊 ：中国中药杂志 2005年 30卷 12期 页码：904.

关键词：大黄；炮制；5种蒽醌苷元；含量测定；

Keywords:rhubarb, processing, five anthraquinones, assay,

摘 要 ：目的:研究炮制对大黄5种蒽醌苷元的影响,建立大黄炮制品质量控制方法。方法:色谱柱填料为Kromasil-C₁₈(4.6mm×150mm,5μm),检测波长254mm。流动相甲醇-0.1%磷酸溶液(85:15)。结果:平均回收率分别为芦荟大黄素97.9%,大黄酸97.1%,大黄素97.6%,大黄酚97.4%,大黄素甲醚99.1%。RSD分别为1.4%,1.1%,0.90%,1.1%,2.2%。生大黄经炮制后,5种蒽醌苷元与生品比都有所下降,分别下降芦荟大黄素15.9%,大黄酸28.0%,大黄素25.8%,大黄酚10.0%,大黄素甲醚10.3%。结论:本方法重复性好,稳定,简单,可用于大黄炮制工艺及质量标准研究。

Abstract:Objective: To investigate the influence of processing of Radix et Rhizoma Rhei on it‘ s five anthraquinone components, and to develop a HPLC quantatitive method for these components in Radix et Rhizoma Rhei. Method: Chromatographic conditions are as follow: Kromasil-C₁₈(4.6 mm· 150 mm,5 μm) column, ethanol -H₂O-H₃PO₄(85:15:0.1) as mobile phase and the detection wavelength at 254 nm. Result: The average recoveries are 97.9% (aleo-emodin), 97.1% (rhein), 97.6% (emodin), 97.4% (chrysophanol) and 99.1% (physcion). RSD are 1.4% , 1.1%, 0.9%, 1.1% and 2.2% correspondingly. After processing, the contents of five anthraquinone agly-cones descended compared with the crude material, the descending percentage are 15.9% (aleo-emodin), 28.0% (rhein), 25.8% (e-modin), 10.0% (chrysophanol) and 10.3% (physcion). Conclusion: This method is simple, sensitive and repeatable. It‘canbeased for quality control of Radix et Rhizoma Rhei and it‘s processed products.

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