Abstract:β-sitosterol and fatty acids were obtained from the oil of seeds of Astragalus complanatus. The fatty acids were separated and their molecular weights determined by GC-MS. Fourteen of them were identified as heptenoic acid, tetradecanoic acid, pentadecanoic acid, hexadecanoie acid, octadecenoic acid, octadecanoic acid, octadecadienoie acid, linolenic acid, eicosanoie acid, eicosenoic acid and docosanoic acid, etc.