Abstract:Foreign insecticidal genes were transferred into 4 upland cotton ( Gossypium hirsutum L. ) cultivars by Agrobacterium-mediated transformation. It was found that planting style has a critical influence on the survival rate of the transgenic plantlets. Direct transplanting, culturing in liquid medium or vermiculite before transplanting were not proper for planting transgenic plantlets. The survival rate was quite low and hardening time was rather long. Graft could greatly enhance the survival rate and efficiently reduced the hardening time, thus paves the way for cotton improvements through genetic engineering and other biotechnology.