Abstract:This paper deals with a new triterpene named triptodihydroxy acid methyl ester, polpunonic acid and wilfortrine were isolated from Tripterygium wilfordii Hook. f. The structure of triptodihydroxy acid methyl ester was identified to be methyl 3β, 30-dihydroxyolean-A12-ene 29 oate. The structure of wilfortrine was elucidated on the basis of MS, IR, UV and NMR spectroscopic analysis. It was shown to be an immunosuppressive and against cells derived from human of leukemia.