Abstract:In order to clarify the change rules and inheritance of Vc content in pepper fruit, fruit Vc content in different pepper genotypes at different maturity levels, and adopted the joint segregation analysis method of major gene plus polygene for Vc content genetic analysis in red ripe fruits were studied. Results showed that the Vc content in the fruit of hot pepper genotypes was significantly higher than in sweet pepper genotypes. And in most varieties, significantly higher Vc content was found in ripe fruit than in mature green fruit, then decreased in the subsequent stages. The Vc content of red fruit was controlled by a pair of additive-dominance major genes and additive-dominance-epistasis polygene (D-0 model), with d value of 18.4276 and h value of-13.3018, indicating the obvious and positive main additive effect and the high-level dominant-negative major genes. The heritability rates for major genes and polygene in F2 generation were 25.97% and 17.13%, respectively. That the acquired genetic variation accounting for 43.1% of phenotypic variation, and environmental variation accounting for the remanent 56.9%, indicating that environmental variation was dominant. This study provided a scientific basis for pepper high quality breeding from the aspect of fruit Vc content.