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作 者 ：李 梅1，田世龙1，颉敏华1，李守强1，张 欣2，程建新1，葛 霞1

期 刊 ：西北植物学报 2012年 8期 页码：1621~1625

关键词：双孢蘑菇；ClO2浸泡处理；褐变；酚类氧化产物；多酚氧化酶；过氧化物酶；

Keywords:Agaricus bisporus, ClO2 treatment, browning, phenolic oxidation products, PPO, POD,

摘 要 ：以‘W2000’双孢蘑菇为试验材料，设清水（CK）和ClO₂浸泡（120 mg·L^-1）2个处理，研究ClO₂浸泡处理在低温贮藏条件下对双孢蘑菇采后品质、生理及相关酶活性的影响。结果显示： ClO₂浸泡处理配合0℃低温贮藏可显著降低双孢蘑菇的呼吸强度，推迟呼吸高峰的出现时间，呼吸强度较CK降低29.2%，并使呼吸高峰推迟5 d出现；同时能够有效抑制子实体多酚氧化酶（PPO）和过氧化物酶（POD）的活性，控制酚类氧化产物的积累，减缓子实体褐化进程，贮藏20 d时，处理的总酚含量仅为0.81 μmol/mg。另外，ClO₂处理还可延缓子实体硬度的下降，保持其可溶性固形物的含量，并抑制开伞，有效延长双孢蘑菇的贮藏期。研究表明，ClO₂处理+低温贮藏对双孢蘑菇具有较理想的保鲜效应，可有效保持双孢蘑菇的外观和营养品质，提高商品价值，具有一定的实际应用价值。

Abstract:An experiment was carried out with the objective of evaluating the effect on postharvest physiology and enzyme activities of Agaricus bisporus of ‘W2000’,with ClO₂ treatment in low temperature storage.This study produced the following results:with ClO₂ treatment and storing at 0℃,respiration rate drops obviously,and the arrival of respiratory climacteric has been postponed,decreased 29.2% and delayed 5 d;Enzyme activities of PPO and POD were significantly suppressed in ClO₂ treated mushrooms,and restrained the accumulation of phenolic oxidation products,as well as slowing the browning rate of fruiting body.Stored 20 d,total phenolic content was only 0.81 μmol/mg.Besides,impregnating with ClO₂ could slow the decreasing speed of hardness of fruiting body,helps soluble solids content to be well maintained,while retarding the cap opening.ClO₂ treatment could effectively extend the storage period,maintain the high quality.Therefore,the commercial value of mushrooms has been greatly improved.In conclusion,the handling of dipping mushrooms in 120 mg·L^-1 ClO₂ solution and stored at 0℃ is very useful in keeping A.bisporus of ‘W2000’ fresh.

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