Abstract:Pathogen inducible promoters are helpful for genetic engineering on disease resistance by restricting expression to infection sites.A 273 bp fragment from the promoter of potato glutathione S transferase gene,named as prp1-1,could mediate rapid and strictly localized transcriptional activation at infection sites.Rs-AFP2 exerts antifungal activity against a broad spectrum of plant pathogenic filamentous fungi.Rs-AFP2 gene under the control of pathogen inducible promoter prp1-1 was introduced into pepper by Agrobacterium tumefaciens-mediated transformation.Reversed transcript PCR results showed that the Rs-AFP2 gene was induced to transcribe only after inoculation with the zoospores of Phytophthora capsici.Tested by root-irrigating with the zoospores of P.capsici,29 of 35 Rs-AFP2 transgenic T1 lines enhanced resistance markedly.Other 23 T1 lines were planted in soil of growth cabinet.And no effect on plant morphology was observed at the Rs-AFP2 transgenic T1 lines.The results suggest the inducible expression of Rs-AFP2 derived by prp1-1 could enhance pepper disease resistance to P.capsici and avoid the adverse effect.