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作 者 ：储忠英,吴丽红,田兰

期 刊 ：中国中药杂志 2014年 39卷 6期 页码：1016.

关键词：荣心丸；五味子醇甲；丹参酮Ⅰ；隐丹参酮；丹参酮ⅡA；五味子乙素；HPLC；

Keywords:Rongxin pills, schizandrin, tanshinone I, cryptotanshinone, tanshinoneⅡA, schizandrin B, HPLC,

摘 要 ：该文通过HPLC建立了同时测定荣心丸（五味子、丹参）中五味子醇甲，丹参酮Ⅰ，隐丹参酮、丹参酮Ⅱ_A，五味子乙素5种有效成分的方法。Agilent C₁₈色谱柱，以甲醇-水为流动相，线性梯度洗脱，体积流量1.0 mL·min^-1；柱温30 ℃；检测波长240 nm。五味子醇甲，丹参酮Ⅰ，隐丹参酮，丹参酮Ⅱ_A，五味子乙素分别在3.000~48.00（r=1.000），3.985~63.76（r=0.999 9），6.370~101.9（r=1.000），8.690~139.0（r=0.999 9），1.700~27.20 mg·L^-1（r=0.999 9）呈良好的线性关系，平均回收率分别为98.44%，100.3%，99.29%，99.07%，98.42%，RSD分别为0.60%，1.1%，0.52%，0.72%，0.97%。实验表明本方法简便、准确、重复性好，可同时测定荣心丸中5种化学成分。

Abstract:To establish a method for determination contents of schizandrin,tanshinone Ⅰ,cryptotanshinone,tanshinoneⅡ_A and schizandrin B in rongxin pills. The HPLC method was performed on an Agilent C₁₈. The mobile phase was composed of methnol and water wish gradient elution. The flow rate was 1.0 mL·min^-1. The column temperature was 30 ℃ and the detection wavelength wash 240 nm. The linear of schizandrin,tanshinone Ⅰ,cryptotanshinone, TanshinoneⅡ_A and schizandrin B were 3.000-48.00(r=1.000), 3.985-63.76(r=0.999 9), 6.370-101.9(r=1.000), 8.690-139.0(r=0.999 9), 1.700-27.20 mg·L^-1(r=0.999 9),respectively. The average recoveries were 98.44%, 100.3%, 99.29%, 99.07%, 98.42%, and RSDs were 0.61%, 1.1%, 0.52%, 0.72%, 0.97%. The method is convenient, accurate and has good precision. It can be used for determination of the preparation.

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