Abstract:This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of chlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, 3,4-dicaffeoylquinic acid, linarin and luteolin in Chrysanthemum indicum. The separation was carried out on a Shim pack VP-ODS(4.6 mm×250 mm, 5 μm) column eluting with mobile phases of methanol (A) and water containing 0.3% phosphoric acid (B) in gradient mode (0-9 min, 85%-80%B; 9-12 min, 80%-70%B; 12-15min, 70%-65%B; 15-20 min, 65%-60%B; 20-23 min, 60%-55%B; 23-29 min, 55%-54.4%B; 29-32 min, 54.4%-45%B; 32-37 min, 45%-5%B ; 37-45 min, 5%-85%B) at the flow rate of 1.0 mL·min-1. The column temperature was 35 ℃ and the detection wavelength was set at 326 nm. The good separation of chlorogenic acid, caffeic acid,1,3-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, 3,4-dicaffeoylquinic acid, linarin and luteolin was achieved within 40 min. Calibration curves of the eight effective components showed good linear relationship(r>0. 999 5,n=7). The average recoveries were within 97.03%-102.3% (RSD<2.0%, n=6). The method is simple, accurate and repeatable and can be used for the quality control of Ch. indicum.