目的：建立多波长同时测定3种獐牙菜属植物中6种活性成分含量的高效液相色谱方法。方法：采用Hypersil BDS色谱柱(4.6 mm×200 mm，5 μm )；流动相为乙腈-0.5%磷酸水溶液,梯度洗脱,流速为1.0 mL·min-1；检测波长为240，274，325，334 nm；柱温25 ℃。结果：在此色谱条件下，各组分在60 min内均得到较好分离；獐牙菜苦苷、龙胆苦苷、去甲当药苷、雏菊叶龙胆苷、去甲基雏菊叶龙胆酮和雏菊叶龙胆酮分别在0.520～20.8，0.202～8.06，0.107～4.28，0.097～3.86，0.094～3.77，0.101～4.02 μg线性良好,相关系数r均为0.999 9，平均回收率分别为98.7%，98.1%，98.3%，98.8%，98.1%，98.6%，RSD均小于3.0%(n=6)。结论：本方法简单、快速、准确，重现性好，可为全面评价和控制獐牙菜属药材质量提供参考。
Objective: To develop an HPLC method for the quantification of six active components in three species (Swertia davidi, S. nervosa and S. mussotii) . Method: The determination was performed on a Hypersil BDS colunm (4.6 mm×200 mm, 5 μm). Acetonitrile and 0.5% phosphoric acid solution were used as the mobile phases with a gradient elution. The flow rate was 1.0 mL·min-1. The UV detection wavelength was at 240, 274, 325 and 334 nm. The column oven temperature was at 25 ℃. Result: Six components were separated commendably in 60 minutes. The calibration curves of swertiamarin, gentiopicroside,norswertianolin,swertianolin, demethylbellidifolin and bellidifolin were in good linearity over the range of 0.520-20.8, 0.202-8.06, 0.107-4.28，0.097-3.86, 0.094-3.77, 0.101-4.02 μg, respectively (r=0.999 9). The average recoveries were 98.7%, 98.1%, 98.3%, 98.8%, 98.1% and 98.6%, respectively, and the RSD were less than 3.0% (n=6). Conclusion: The method is accurate,simple and reproducible, and can be used to control the quality of Swertia