Abstract:Objective: To constitute HPLC fingerprint of the methanol extract from Swertia mussotii grown in Sichuan Province. Mxethod: RP-HPLC, methanol and water including 0.02% acid as mobile phase, gradient elution, flow rate 1.0 mL·min-1,the detection wavelength was 260 nm, temperature was 30 ℃. Result: The RSD values of peak area and retention time of common peaks in precision, repeatability and stability were lower than 5.0%, respectively, similarity was over 0.805 in S. mussotii collected from 10 different habitats. Conclusion: All results above exhibit that this method is simple, practicable, and reliable as a standard method in controlling the quality of S. mussotii.