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期 刊 ：中国中药杂志 2002年 27卷 12期 页码：913.

关键词：HPLC法；生发散；何首乌；4-四羟基二苯乙烯-2-O-β-D-葡萄糖苷；

Keywords:RP-HPLC, Shengfa Powders, Radix Polygoni Multif lori, 2, 3, 5, 4‘-tetrahydroxy-stilbene-2-O-β-D-glycoside,

摘 要 ：目的:建立一种测定生发散中何首乌特征成分2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的方法。方法:采用Hypersil BDS C₁₈柱(4.6mm×150mm,5μm);流动相为乙腈-水(18:82);紫外检测波长为320nm。结果:线性范围为0.45-2.25μg,r=0.999 8,回收率99.5％,RSD为1.2％(n=6)。结论:本法测定生发散中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量,操作简便,结果准确、可靠。

Abstract:Objective: To develop a new method for the determination of the idiosyncratic component, 2,3,5,4‘- tetrahydrox-y-stilbene-2-O-β-D-glycoside in Shengfa Powders. Method: A HPLC method was set up, using Hypersil BDS C₁₈ column (5μm, 4.6 mm×150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm. Result: The calibration curve was linear in the range of 0.45-2. 25μg, r=0.9998, the average recovery was 99.5% , RSD=1.2% (n=6). Conclusion: The active constituent 2,3,5,4‘- tetrahydroxy-stilbene-2-O-β-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.

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