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作 者 ：陈艳成1,吴 韬1,刘海峰2,肖向文1,李晓波1*

期 刊 ：西北植物学报 2012年 5期 页码：1047~1051

关键词：棕色棉；花色苷；紫外-可见吸收光谱分析；HPLC；

Keywords:brown-fiber cotton, anthocyanins, UV absorption spectrum, HPLC,

摘 要 ：以新疆深棕色棉品系‘棕86’的成熟纤维为材料，用甲醇溶液(V_MeOH∶V_HCl=99∶1)避光冷浸提取15 d后，浓缩成浸膏。用适量蒸馏水溶解浸膏，乙酸乙酯萃取除杂，水相用HP 20大孔树脂纯化色素组分；采用紫外 可见吸收光谱分析，并结合高效液相色谱方法，对天然彩色棉纤维色素组分进行鉴定，为彩色棉花新色彩的分子改良提供参考。结果显示，棕色棉纤维提取液中含有花色苷，且至少含有4种不同的花色苷成分。对棕色棉纤维提取液中花色苷的降解分析显示，棕色棉中的色素物质很不稳定。研究表明，花色苷是棕色棉纤维色素中的一种重要组成成分，进一步补充了彩棉色素的物质基础。

Abstract:Composition and characterization of fiber pigments in one brown-fiber cotton cultivar (line) (Gossypium hirsutum L.) ‘Zong 86’ was studied in this research.The fiber pigments were extracted for 15 d by methanol solution (V_MeOH∶V_HCl=99∶1) at room temperature in the dark.The methanol extract was evaporated to dryness and the residue was dissolved in distilled water.The aqueous solution was extracted with ethyl acetate and then the water phase was used to purify pigments by HP-20 macroporous resin separation.The pigment composition and characterization was analyzed by UV absorption spectrum and HPLC method.The results showed that the pigment extract from the brown cotton fibers contained at least four types of anthocyanins.This study indicated that the anthocyanins were one important component of the pigments in brown-colored cotton fibers,which provides the clues and basis for improving fiber-color character of naturally colored cotton.

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