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期 刊 ：中国中药杂志 2008年 33卷 15期 页码：1890.

关键词：东莨菪素；在体胃肠吸收；高效液相色谱法；

Keywords:scopoletin, in situ absorption from stomach and intestine, HPLC,

摘 要 ：目的：研究东莨菪素在大鼠胃、小肠的吸收特性。方法：采用大鼠在体胃、小肠吸收实验模型，以高效液相色谱法测定灌注液中药物的浓度，紫外分光光度法测定酚红的浓度。结果：东莨菪素在大鼠胃中2 h的吸收百分率为76.31%；东莨菪素在结肠、十二指肠、回肠、空肠的吸收率分别为46.25%，40.54%，38.21%，32.77%；不同质量浓度东莨菪素(10.014 4，20.028 8，40.057 6 mg·L^-1在小肠的吸收速率常数分别为0.643 4，0.613 7，0.597 0 h^-1；不同pH(6.0，6.8，7.4)在小肠的吸收速率常数分别为0.621 7，0.603 3，0.613 7 h^-1。结论：不同质量浓度、pH对东莨菪素在大鼠全肠道的吸收没有显著性影响，药物的吸收呈一级动力学过程，吸收机制主要为被动扩散；东莨菪素在胃肠道均有较好的吸收，结肠为最佳吸收部位，提示适宜制成缓控释制剂。

Abstract:Objective: To study absorption kinetics of scopoletin in rat stomachs and intestines. Method: Rats was cannulated for in situ recirculation. UV and HPLC methods were used to determine the concentrations of phenolsulfonphthalein and scopoletin, respectively. Result: The absorption rates in rat stomachs at 2 h after administration was 76.31%; The absorption rates at colon, duodenum, ileum and jejunum were 46.25%, 40.54%, 38.21%, 32.77%, respectively. The absorption rate constant (K_a) at concentrations of 10.014 4, 20.028 8-40.057 6 mg·L^-1 in intestine were 0.643 4, 0.613 7, 0.597 0 h^-1, respectively. The K_a of scopoletin at pH of 6.0, 6.8 and 7.4 in intestine were 0.621 7, 0.603 3, 0.613 7 h^-1, respectively. Conclusion: The concentrations and pH values of scopoletin solution had no distinctive effect on the absorption kinetics. The absorption of scopoletin was a first-order process with passive diffusion mechanism. Scopoletin was well absorbed at stomachs and intestines in rats. Colon was the best absorption site of scopoletin, which suggest that a sustained-release preparation should be suitable for this compound.

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